Evaluating phase separation in live cells: diagnosis, caveats, and ...
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By dt mcswiggen · 2019 · cited by 314 — the idea that liquid–liquid phase separation (llps) may ... a single image or movie, whereas few (six and eight exam- ples out of 50, respectively) measure ...
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PERSPECTIVEEvaluatingphaseseparationinlivecells:diagnosis,caveats,andfunctionalconsequencesDavidT.McSwiggen,1,2MustafaMir,1XavierDarzacq,1,2andRobertTjian1,31DepartmentofMolecularandCellBiology,UniversityofCaliforniaBerkeley,California94720,USA;2CaliforniaInstituteofRegenerativeMedicineCenterofExcellence,UniversityofCaliforniaBerkeley,California94720,USA;3HowardHughesMedicalInstitute,UniversityofCaliforniaBerkeley,California94720,USATheideathatliquid–liquidphaseseparation(LLPS)maybeageneralmechanismbywhichmoleculesinthecom-plexcellularmilieumayself-organizehasgeneratedmuchexcitementandfervorinthecellbiologycommunity.Whilethisconceptisnotnew,itsrisetopreeminencehasresultedinrenewedinterestinthemechanismsthatshapeanddrivediversecellularself-assemblyprocessesfromgeneexpressiontocelldivisiontostressresponses.Invitrobiochemicaldatahavebeeninstrumentalinderiv-ingsomeofthefundamentalprinciplesandmoleculargrammarbywhichbiologicalmoleculesmayphasesepa-rate,andthemolecularbasisoftheseinteraction...
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