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Sarstedt, Inc. Cell Culture Application Note Protein-coated Tissue Culture Surfaces Improve Attachment, Proliferation and Differentiation in Commonly Used Cell Lines Aaron Haubner, Ph.D. Sarstedt, Inc. Newton, NC INTRODUCTION In their native environment cells are connected through a scaffold of adhesion proteins and glycosaminoglycans known as the extracellular matrix (ECM)1. Cellular attachment to a growth surface is a complex interaction involving a variety of surface adhesion receptors, and it affects cellular functions like proliferation, apoptosis, protein expression, invasion, and differentiation among others, primarily via effects on the cytoskeleton2,3,4. While most in-vitro cell cultures have been adapted over time to grow on traditional surfaces like glass and treated plastic, some cell types including weakly adherent cells, primary cells, stem cell lines, and some transfected cells benefit from a robust growth surface that better mimics the native ECM5. Because of this, cell...