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ARTICLELong-termimagingofdorsalrootgangliainawakebehavingmiceChaoChen1,5,JinhuiZhang2,5,LinlinSun3,5,YilingZhang1,Wen-BiaoGan4,PeifuTang1&GuangYang3Thedorsalrootganglia(DRG)containthesomasoffirst-ordersensoryneuronscriticalforsomatosensation.Duetotechnicaldifficulties,DRGneuronalactivityinawakebehavinganimalsremainsunknown.Here,wedevelopamethodforimagingDRGatcellularandsubcellularresolutionoverweeksinawakemice.Themethodinvolvestheinstallationofanintervertebralfusionmounttoreducespinalmovement,andtheimplantationofavertebralglasswindowwithoutinterferinganimals’motorandsensoryfunctions.Invivotwo-photoncalciumimagingshowsthatDRGneuronalactivityishigherinawakethananesthetizedanimals.Immediatelyafterplantarformalininjection,DRGneuronalactivityincreasessub-stantiallyandthisactivityupsurgecorrelateswithanimals’phasicpainbehavior.RepeatedimagingofDRGover5weeksafterformalininjectionrevealspersistentneuronalhyper-activityassociatedwithongoingpain.Themethoddescribedhereprovidesanimportantmeansforinv...