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eSPC, an Online Data AnalysisPlatform for MolecularBiophysicsPhotoMol User DocumentationFebruary 2022 Table of Contents1. Analysis1.1. Fitting model1.2. Input file1.3. Bin width1.4. Minimum observed mass1.5. Starting values1.6. Upper limit for the standard deviation1.7. Tolerance to the initial guesses1.8. Window range1.9. Baseline1.10. Curve fitting2. Calibration OverviewPhotoMolwasdevelopedtoestimatethemassesofdifferentspeciesinasampleafteraMassPhotometryexperiment.Moredetailsaboutthistechnologycanbefound athttps://www.refeyn.com/.1. Analysis1.1. Fitting modelThe function that we use to fit is a sum of truncated Gaussians(1)𝑦=𝑏 + 𝑖=1π‘›βˆ‘π‘”π‘–(π‘₯)whereyrepresents the histogram counts,xthe masses,nis the number of truncatedGaussiansg(x),bis a user-defined baseline, andg(x)is defined as follows.(2)𝑔(π‘₯)=π‘Žπ‘šπ‘*𝑒π‘₯𝑝(βˆ’(π‘₯βˆ’π‘π‘’π‘›π‘‘π‘’π‘Ÿ)2 2Οƒ2) 𝑖𝑓 π‘₯ β‰₯ π‘₯π‘‘β„Žπ‘Ÿπ‘’π‘ β„Žπ‘œπ‘™π‘‘ 𝑒𝑙𝑠𝑒 0wherexthresholdistheminimumvalueofx(mass)thatcanbeobserved,centeristhecenter of the gaussian,is the standa...