The effects of x-irradiation in solution on the kinetic, sedimentation and ...

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By sj adelstein · 1961 · cited by 24 — arch. biochem. biophys. 88, 275. krishnamurthy, s., seshadri sastry, p. & ganguly, j. (1958). arch. biochem. biophys. 75, 6.

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406P.SESHADRISASTRYANDJ.GANGULY1961iodoaceticacidinhibitedthevitaminAesteraseandcholesterolesterase,butnotthecommonesterase.4.ThevitaminAesteraseofratliverwasmorelabiletowardsagingandheat,andtheothertwoesterasesweremorestable.Acetone-dryingat0-50preferentiallydestroyedvitaminAesteraseandcholesterolesterase,butnotthecommonesterase.5.ThevitaminAesteraseofnuclearandmicro-somalfractionsofchickenliverhadsimilarpHoptimaatpH6-6and8-6,withmaximalactivityatpH8-6,andtheirK.valueswere0-19and0-33mrespectively.ThecholesterolesteraseofbothsourceshadthesamepHoptimumatpH8-2,andtheirKmvalueswere0-28and0-15mMrespectively.6.Thethreeesteraseactivitiesofthenuclear,butnotofthemicrosomal,preparationfromchickenliverweresolubilizedbyLissapol-N;thedetergentactivatedthenuclearcholesterolesterase,butnotthevitaminAesterase.7.AmmoniumsulphatefractionationoftheLissapolextractgave66-6%oftheoriginalvitaminA-esteraseactivityinthefractionbetween28-5and37-5%saturation,whereas60%ofthecommonesterasewasrecoveredfromthefract...

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